Abstract
Fungal endophytes associated with the oil palm, Elaeis guineensis, at an oil palm plantation in Trang Province, Thailand were investigated. Two samplings at the same site yielded 162 and 178 morphotypes. The overall colonization rate was 81 and 87%, respectively, with non-sporulating species being most common. Twenty strains were identified as basidiomycetes using morphological characteristics and confirmed at the molecular level using ribosomal DNA, LSU and ITS sequence data. Species included Fomitopsis cf. meliae, F. cf. ostreiformis, F. cf. pinicola, Perenniporia sp., Pycnoporus sanguineus, Trametes lactinea, and Schizophyllum commune. Leaves supported the greatest number of basidiomycetous taxa (11 strains) with five and four taxa from petioles and rachides, respectively. Twelve taxa produced poroid basidiomes in PDA culture, while the remainder had clamp-connections. Basidiomycetes occurring as saprobes on oil palm were also recorded and compared with those present as endophytes.
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References
Arnold AE (2007) Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biol Rev 21:51–66
Arnold AE, Lutzoni F (2007) Diversity and host range of foliar fungal endophytes: are tropical leaves biodiversity hotspots? Ecology 88:541–549
Arnold AE, Mejía L, Kyllo D, Rojas E, Maynard Z, Herre EA (2003) Fungal endophytes limit pathogen damage in a tropical tree. Natl Acad Sci USA 100:15649–15654
Arnold AE, Henk DA, Eells RL, Lutzoni F, Vilgalys R (2007) Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and enviormental PCR. Mycologia 99:185–206
Azlin CO, Amir HG, Chan LK (2005) Isolation and characterization of diazotrophic rhizobacteria of oil palm roots. Malaysian J Microbiol 1:31–35
Bidartondo MI, Bruns TD, Weiss M, Sergio C, Read DJ (2003) Specialized cheating of the ectomycorrhizal symbiosis by an epiparasitic liverwort. Proc R Soc Lond: Biol Sci 270:835–842
Bills GF (1996) Isolation and analysis of endophytic fungal communities from wood plants. In: Carris LM, Redlin SC (eds) Endophytic fungi in grasses and woody plants. APS Press, St. Paul, pp 31–65
Binder M, Hibbett DS, Wang Z, Farnham WF (2006) Evolutionary relationships of Mycaureol dilseae (Agaricales), a basidiomycetes pathogen of a subtidal Rhodophyte. Am J Bot 93:547–556
Bougher NL, Lebel T (2001) Sequestrate (Truffle-like) fungi of Australia and New Zealand. Aust Syst Bot 14:439–484
Bougoure JJ, Bougoure DS, Cairney JWG, Dearnaley JDW (2005) ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (Orchidaceae) in southern Queensland. Mycol Res 109:452–460
Bunyard BA, Nicholson MS, Royse DJ (1994) A systematic assessment of Morchella using RFLP analysis of the 28S ribosomal RNA gene. Mycologia 86:762–722
Cannon PF, Simmons GM (2002) Diversity and host preference of leaf endophytic fungi in the Iwokrama Forest Reserve, Guyana. Mycologia 94:210–220
Carroll G (1988) Fungal endophytes in stems and leaves: from latent pathogen to mutalistic symbiont. Ecology 69:2–9
Chapela IH, Boddy L (1988a) Fungal colonization of attached beech branches. I. Early stages of development of fungal communities. New Phytol 110:39–45
Chapela IH, Boddy L (1988b) Fungal colonization of attached beech branches. II. Spatial and temporal organization of communities arising from latent invaders in bark and functional sapwood, under different moisture regimes. New Phytol 110:47–57
Choeyklin R (2009) The ecology and lignocellulose degrading ability of basidiomycetes on selected bamboo and palms. Ph.D. Thesis. Burapha University, Thailand
Choeyklin R, Hattori T, Jarikhuan S, Jones EBG (2009) Bambusicolous poplypores collected in Central Thailand. Fungal Divers 36:121–128
Clay K (1989) Clavicipitaceous endophytes of grasses: their potential as biocontrol agents. Mycol Res 92:1–12
Corner RJH (1989a) Ad Polyporaceas V. The genera Albatrellus, Boletopsis, Coriolopsis (dimitic), Cristelloporia, Diacanthodes, Elmerina, Fomitopsis (dimitic), Gloeoporus, Grifolia, Hapalopilus, Heterobasidion, Hydnopolyporus, Ischnoderma, Loweporus, Parmastomyces, Perenniporia, Pyrofomes, Stecchericium, Trechispora, Truncospora and Tyromyces. Nova Hedwigia Heft 96:1–218
Corner RJH (1989b) Ad Polyporaceas VI. The genus Trametes. Nova Hedwigia Heft 97:1–197
Crozier J, Thomas SE, Aime MC, Evans HC, Holmes KA (2006) Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol 55:783–791
Currah RS, Sherburne R (1992) Septal ultrastructure of some fungal endophytes from boreal orchid mycorrhizas. Mycol Res 96:583–587
D’Amico M, Frisullo S, Cirulli M (2008) Endophytic fungi occurring in fennel, lettuce, chicory, and celery – commercial crops in southern Italy. Mycol Res 112:100–107
Duong LM, Jeewon R, Lumyong S, Hyde KD (2006) DGGE coupled with ribosomal DNA phylogenies reveal uncharacterized fungal phylotypes on living leaves of Magnolia liliifera. Fungal Divers 23:121–138
Duckett JG, Russell J, Ligrone R (2006) Basidiomycetous endophytes in jungerman-nialean (leafy) liverworts have novel cytology and species-specific host ranges: a cytological and experimental study. Can J Bot 84:1075–1093
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797
Evans HC, Holmes KA, Thomas SE (2003) Endophytes and mycoparasites associated with an indigenous forest tree, Theobroma gileri, in Ecuador and preliminary assessment of their potential as biocontrol agents of cocoa diseases. Mycol Progr 2:149–160
Eziashi EI, Omamor IB, Dimaro-Oruade EA, Ogunkanmi LA (2007) Control of phytotoxin from Ceratocystis paradoxa using Trichoderma species phytotoxins on oil palm (Elaeis guineensis Jacq.) sprouted seeds. Plant Pathol J 6:324–329
Fischer PJ, Petrini O, Sutton BC (1994) Fungal endophytes from the leaves and twigs of Quercus ilex L. from England, Majorca, and Switzerland. New Phytol 127:133–137
Fröhlich J, Hyde KD (1999) Biodiversity of palm fungi in the tropics; are global fungal diversity estimates realistic? Biodivers Conserv 8:977–1004
Fröhlich J, Hyde KD, Petrini O (2000) Endophytic fungi associated with palms. Mycol Res 104:1202–1212
Gamboda MA, Bayman P (2001) Communities of endophytic fungi on leaves of a tropical timber tree (Guarea guidonia: Meliaceae). Biotropica 33:352–360
Giordano L, Gonthier P, Varese GC, Miserere L, Nicolotti G (2009) Mycobiota inhabiting sapwood of healthy and declining Scots pine (Pinus sylvestris L.) trees in the Alps. Fungal Divers 38:69–83
Gomez-Vidal S, Lopez-Llorca LV, Jansson HB, Salinas J (2006) Endophytic colonization of date palm (Phoenix dactylifera L.) leaves by entomopathogenic fungi. Micron 37:624–632
Guo LD, Hyde KD, Liew ECY (2001) Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequence. Mol Phylogenet Evol 20:1–13
Hall T (2006) BioEdit. Version 7.5.0.3. Department of Microbiology, North Carolina State University
Hattori T, Lee SS (1999) Two new species of Perenniporia described from a lowland rainforest of Malaysia. Mycologia 91:525–531
Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lucking R, Thorsten Lumbsch H, Lutzoni F, Matheny PB, McLaughlin DJ, Powell MJ, Redhead S, Schoch CL, Spatafora JW, Stalpers JA, Vilgalys R, Aime MC, Aptroot A, Bauer R, Begerow D, Benny GL, Castlebury LA, Crous PW, Dai YC, Gams W, Geiser DM, Griffith GW, Gueidan C, Hawksworth DL, Hestmark G, Hosaka K, Humber RA, Hyde KD, Ironside JE, Koljalg U, Kurtzman CP, Larsson KH, Lichtwardt R, Longcore J, Miadlikowska J, Miller A, Moncalvo JM, Mozley-Standridge S, Oberwinkler F, Parmasto E, Reeb V, Rogers JD, Roux C, Ryvarden L, Sampaio JP, Schussler A, Sugiyama J, Thorn RG, Tibell L, Untereiner WA, Walker C, Wang Z, Weir A, Weiss M, White MM, Winka K, Yao YJ, Zhang N (2007) A higher-level phylogenetic classification of the Fungi. Mycol Res 111:509–547
Ho YW, Varghese G (2008) Pathogenic potential of soil Fusaria from Malaysian oil palm habitats. J Phytopathol 115:325–331
Hoff JA, Klopfenstein NB, McDonald GI, Tonn JR, Kim MS, Zambino PJ, Hessburg PF, Rogers JD, Peever TL, Carris LM (2004) Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa). Forest Pathol 34:255–271
Hoffman MT, Arnold AE (2008) Geographic locality and host identity shape fungal endophyte communities in cupressaceous tress. Mycol Res 112:231–234
Huang WY, Cai YZ, Hyde KD, Corke H, Sun M (2008) Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Divers 33:61–75
Huang WY, Cai YZ, Surveswaran S, Hyde KD, Corke H, Sun M (2009) Molecular phylogenetic identification of endophytic fungi isolated from three Artemisia species. Fungal Divers 36:69–88
Huelsenbeck JP, Ronquist F (2001) Mr.Bayes: Bayesian inference of phylogenetic diversity. Bioinformatics (Oxford) 17:754–755
Hyde KD, Soytong K (2008) The fungal endophyte dilemma. Fungal Divers 33:163–173
Jones EBG, Alias SA, Nawawi A (2007) New fungi described from Malaysia. In: EBG Jones, KD Hyde, S Vikineswary (eds) Malaysian fungal diversity. Mushroom Research Centre, University of Malaya, and Ministry of Natural Resources and Environment Malaysia, pp 379–419
Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Anisworth & Bisby’s Dictionary of The Fungi, 10th edn. CAB International: Wallingford, pp 771
Kottke I, Beiter A, Weiss M, Haug I, Oberwinkler F, Nebel M (2003) Heterobasidiomycetes form symbiotic associations with hepatics: Jungermanniales have sebacinoid mycobionts while Aneura pinguis (Metzgeriales) is associalted with a Tulasnella species. Mycol Res 107:957–968
Kristiansen KA, Fredenstein JV, Rasmussen FN, Rasmussen HN (2004) Molecular identification of mycorhizal fungi in Neuwiedia veratrifolia (Orchidaceae). Mol Phylogenet Evol 33:251–258
Lacap DC, Hyde KD, Liew ECY (2003) An evaluation of the fungal “morphotype” concept on ribosomal DNA sequence. Fungal Divers 12:53–66
Lee BKH, Baker GE (1972) An ecological study of the microfungi in Hawaiian mangrove swamp. Pac Sci 26:1–19
Ludley KE, Robinson CH (2008) Decomposer Basidiomycota in Arctic and Antarctic ecosystems. Soil Biol Biochem 40:11–29
Lumyong S, Lumyong P, Hyde KD (2004). Endophytes. In: EBG Jones, M Tanticharoen, KD Hyde (eds) Thai fungal diversity. BIOTEC Thailand: pp 197–205
Lumyong S, Techa W, Lumyong P, McKenzie EHC, Hyde KD (2009) Endophytic fungi from Calamus kerrianus and Wallichia caryotoides (Arecaceae) at Doi Suthep-Pui National Park, Thailand. Chiang Mai J Sci 36:158–167
Maria GL, Sridhar KR (2003) Endophytic fungal assemblage of two halophytes from west coastal mangrove habitats, India. Czech Mycol 55:241–251
O’Donnell K, Cigelnik E, Weber NS, Trappe JM (1997) Phylogenetic relationship among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis. Mycologia 89:48–65
Okane I, Srikitikulchai P, Toyama K, Læssøe T, Sivicahi S, Hywel-Jones N, Nakagiri A, Potacharoen W, Suzuki KI (2008) Study of endophytic Xylariaceae in Thailand: diversity and taxonomy inferred from rDNA sequence analyses with saprobes forming fruit bodies in the field. Mycoscience 49:359–372
Oses R, Valenzuela S, Freer J, Sanfuentes E, Rodríguez J (2008) Fungal endophytes in xylem of healthy Chilean trees and their possible role in early wood decay. Fungal Divers 33:77–86
Paterson RPM (2007) Ganoderma disease of oil palm-A white rot perspective necessary for integrated control. Crop Projection 26:1369–1376
Pažoutová S, Šrůtka P (2007) Symbiotic relationship between Cerrena unicolor and the horntail Tremex fuscicornis recorded in the Czech Republic. Czech Mycol 59:83–90
Promputtha I, Jeewon R, Lumyong S, McKenzie EHC, Hyde KD (2005) Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae). Fungal Divers 20:167–186
Puri SC, Asiya N, Raman C, Rajesh A, Hasan R, Touseef A, Bilal A, Vijeshwar V, Shinkha S, Ravinder S, Ashok S, Raj K, Sharma RK, Qazi GN (2006) The endophytic fungus Trametes hirsuta as a novel alternative source of podophyllotoxin and related aryl tetralin lignans. J Biotechnol 122:494–510
Raghukumar C (2008) Marine fungal biotechnology: an ecological perspective. Fungal Divers 31:19–35
Rodrigues KF (1994) The foliar fungal endophytes of the Amazonian palm Euterpe oleracea. Mycologia 86:376–385
Rodrigues KF, Samuels GJ (1990) Preliminary study of endophytic fungi in a tropical palms. Mycol Res 94:827–830
Rungjindamai N, Pinruan U, Cheyklin R, Hattori T, Jones EBG (2008) Molecular characterization of basidiomycetous endophytes isolated from leaves, rachis and petioles of the oil palm, Elaeis guineensis, in Thailand. Fungal Divers 33:139–161
Sánchez Márquez S, Bills GF, Zabalgogeazcoa I (2007) The endophytic mycobiota of the grass Dactylis glomerata. Fungal Divers 27:171–195
Sánchez Márquez S, Bills GF, Zabalgogeazcoa I (2008) Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Divers 33:87–100
Sapak Z, Meon S, Ahmad ZAM (2008) Effect of endophytic bacteria on growth and suppression of Ganoderma Infection in oil palm. Int J Agric Biol 10:127–132
Southcott KA, Johnson JA (1997) Isolation of endophytes from two species of palm from Bermuda. Can J Microbiol 43:789–792
Suryanarayanan TS, Thirumalai E, Prakash CP, Govinda Rajulu MB, Thirunavukkarasu N (2009) Fungi from two forests of southern India: a comparative study of endophytes, phellophytes, and leaf litter fungi. Can J Microbiol 55:419–426
Swofford DL (2002) PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods) Version 4.0b10. Sinauer Associates, Sunderland, MA
Tao G, Liu ZY, Hyde KD (2008) Whole rDNA analysis reveals novel and endophytic fungi in Bletilla ochraea (Orchidaceae). Fungal Divers 33:101–122
Taylor JE, Hyde KD, Jones EBG (1999) Endophytic fungi associated with the temperate palm, Trachycarpus fortunei, within and outside its natural geographical range. New Phytol 142:335–346
Thomas SE, Crozier J, Aime MC, Evans HC, Holmes KA (2008) Molecular characterization of fungal endophytic morphospecies associated with the indigeneous forest tree, Theobroma gileri, in Ecuador. Mycol Res 112:852–860
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Tomšovsky M (2008) Molecular phylogeny and taxonomic position of Trametes cervina and description of a new genus Trametopsis. Czech Mycol 60:1–11
Tomšovsky M, Kolarik M, Pazoutova S, Homolka L (2006) Molecular phylogeny of European Trametes (Basidiomycetes, Polyporales) species based on LSU and ITS (nrDNA) sequences. Nova Hedwigia 82:269–280
Treu R (1998) Macrofungi in oil palm plantations of South East Asia. Mycologist 12:10–14
Umali TE, Quimio TH, Hyde KD (1999) Endophytic fungi in leaves of Bambusa tuldoides. Fungal Sci 14:11–18
Waller F, Achatz B, Baltruschat H, Fodor J, Becker K, Fischer M, Heier T, Hückelhoven R, Neumann C, von Wettstein D, Franken P, Kogel KH (2005) The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proc Natl Acad Sci 102:13386–13391
Wang Y, Guo LD, Hyde KD (2005) Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in notheast China based on rDNA sequences. Fungal Divers 20:235–260
Warcup JH (1991) The Rhizoctonia endophytes of Rhizanthella (Orchidaceae). Mycol Res 95:656–641
Weiss M, Selosse MA, Rexer KH, Urban A, Oberwinkler F (2004) Sebacinales: a hitherto overlooked cosm of heterobasidiomycetes with a broad mycorrhizal potential. Mycol Res 108:1003–1010
White TF, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA gene for phylogenetics. In: Innis MA, Gelfand DH, Sninsky FS, White TT (eds) PCR protocal: a guide to methods and applications. Academic, San Diego, pp 315–322
Zhu GS, Yu ZN, Gui Y, Liu ZY (2008) A novel technique for isolating orchid mycorrhizal fungi. Fungal Divers 33:123–137
Zuccaro A, Schulz B, Mitchell JI (2003) Molecular detection of ascomycetes associated with Fucus serratus. Mycol Res 107:1451–1466
Zuccaro A, Summerbell RC, Gams W, Schroers H-F, Mitchell JI (2004) A new Acremonium species associated with Fucus spp, and its affinity with a phylogenetically distinct marine Emericellopsis clade. Stud Mycol 50:283–297
Zuccaro A, Schoch CL, Spatafora JW, Kohlmeyer J, Draeger S, Mitchell JI (2008) Detection and identification of fungi intimately associated with the brown seaweed Fucus serratus. Appl Environ Microbiol 74:931–941
Acknowledgements
This project was supported by the Thailand Research Fund (TRF) and National Center for Genetic Engineering and Biotechnology (BIOTEC) under special program for Biodiversity Research and Training Program (Grant numbers: BRT_R150004, BRT_R650001, and BRT_R352024) and Thailand Graduate Institute of Science and Technology grant (TG-22-10-49-019D). We are grateful to the Graduate School, Chiang Mai University. We thank the following for their comments on the identification of the Trametes species and their geographical distribution: Drs. Tsutomu Hattori, Karen Hughes, Ron Petersen, Michal Tomšovsky, and J. Vlasak. Anupong Klaysuban is thanked for technical support. We thank Drs. Kanyawim Kirtikara, Lily Eurwilaichitr and Morakot Tanticharoen, BIOTEC, for their constant interest and support.
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Pinruan, U., Rungjindamai, N., Choeyklin, R. et al. Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand. Fungal Diversity 41, 71–88 (2010). https://doi.org/10.1007/s13225-010-0029-1
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DOI: https://doi.org/10.1007/s13225-010-0029-1