The objective of this study was to identify and study the effect of colchicine treatment on the growth and enzyme production of Pycnoporus coccineus KKUPN1. Molecular identification using universal primer ITS1 and ITS4 found that KKUPN1 was Pycnoporus coccineus (ID: KU202741). The effect of colchicine when culturing P. coccineus KKUPN1 in Czapex doc broth and potato dextrose broth (PDB) containing 0, 0.05 and 0.1% (v/v) colchicine for 7 and 14 d was studied. The result showed that in the early stage all treatments containing colchicine gave better growth than the treatments without colchicine. However, 5 d after inoculation, the growth rates were not significantly different in all treatments. The colony morphology of P. coccineus KKUPN1 isolated from Czapex doc broth containing colchicine showed the production of orange-red pigment that was not present from the medium without colchicine. In PDB containing 0.05 and 0.1% (v/v) colchicine for 7 d, the colonies had orange-red pigment from the edge of the colony to the center, while 0% (v/v) colchicine produced sparse mycelia with slight pigment. The enzyme test of P. coccineus KKUPN1 cultured in Czapex doc broth or PDB with colchicine added produced results that showed they did not produce a hemicellulolytic enzyme and a lignin modification enzyme, but could produce a cellulolytic enzyme that was detected by CMC agar and esculin agar but could not be detected by cellulose agar. Keywords: Colchicine, Enzyme production, Pycnoporus coccineus.